Homogeneous batch micro-crystallization of proteins from ammonium sulfate

Preparation of High-Purity Grade Ammonium Sulfate from Locally Available Products

Semi-batch Crystallization of Gypsum from Calcite and Sulfuric Acid

One of the most important environmental issues for decades to come is the treatment of industrial wastewaters containing salts (sulfates, chlorides, sodium, magnesium ...) which can lead to strong perturbations affecting the fauna and flora when highly concentrated at the discharge point. Different processes used to separate the dissolved salts from the liquid matrix may be encountered dependin...

Ammonium sulfate – malonic acid aerosols

A study of the phase transition behavior of mixed ammonium sulfate – malonic acid aerosols C. F. Braban and J. P. D. Abbatt Centre for Atmospheric Sciences, Department of Chemistry, Lensfield Road, University of Cambridge, Cambridge, CB2 1EW, UK Department of Chemistry, University of Toronto, Toronto, ON, M5S 3H6, Canada Received: 22 March 2004 – Accepted: 3 May 2004 – Published: 2 June 2004 Co...

Crystallization of chicken egg white lysozyme from assorted sulfate salts

Chicken egg white lysozyme has been found to crystallize from ammonium, sodium, potassium, rubidium, magnesium, and manganese sulfates at acidic and basic pH, with protein concentrations from 60 to 190 mg/ml. Crystals have also been grown at 4°C in the absence of any other added salts using isoionic lysozyme which was titrated to pH 4.6 with dilute sulfuric acid. Four different crystal forms ha...

Glutamate decarboxylase from Lactobacillus brevis: activation by ammonium sulfate.

In this study, the glutamate decarboxylase (GAD) gene from Lactobacillus brevis IFO12005 (Biosci. Biotechnol. Biochem., 61, 1168-1171 (1997)), was cloned and expressed. The deduced amino acid sequence showed 99.6% and 53.1% identity with GAD of L. brevis ATCC367 and L. lactis respectively. The His-tagged recombinant GAD showed an optimum pH of 4.5-5.0, and 54 kDa on SDS-PAGE. The GAD activity a...